INTEGRATED OPTICAL OUTPUT GRATING COUPLER AS BIOCHEMICAL SENSOR

Authors
CLERC D LUKOSZ W
Citation
D. Clerc et W. Lukosz, INTEGRATED OPTICAL OUTPUT GRATING COUPLER AS BIOCHEMICAL SENSOR, Sensors and actuators. B, Chemical, 19(1-3), 1994, pp. 581-586
Citations number
1
Categorie Soggetti
Engineering, Eletrical & Electronic","Instument & Instrumentation
Journal title
Sensors and actuators. B, Chemical → ACNP
ISSN journal
09254005
Volume
19
Issue
1-3
Year of publication
1994
Pages
581 - 586
Database
ISI
SICI code
0925-4005(1994)19:1-3<581:IOOGCA>2.0.ZU;2-4
Abstract
The output-grating-coupler sensor was used for real-time studies of th e adsorption of the proteins avidin, NeutrAvidin, and streptavidin. Th e feasibility of affinity sensing with subnanomolar detection limit wa s demonstrated with biotin-BSA (bovine serum albumin) as analyte bindi ng to immobilized (adsorbed) avidins on the waveguide surface. Not onl y the surface coverage but also both the thicknesses and refractive in dices of the adsorbed and bound adlayers were determined.