PERFORMANCE OF INTEGRATED GLUCOSE AND LACTATE THIN-FILM MICROBIOSENSORS FOR CLINICAL ANALYZERS

Integrated thin-film glucose and lactate sensors were produced using t hin photo-patternable multi-enzyme membranes. The glucose and lactate sensors are based on the measurement of H2O2 on a Pt electrode produce d by the enzymes glucose oxidase (GOD) and lactate oxidase (LOD), resp ectively, which were entrapped in photo-patterned pHEMA hydrogel membr anes. To prevent electrochemical interferences and fouling of the Pt e lectrode an electropolymerized semipermeable membrane was used. A seco nd enzyme membrane layer containing the enzyme catalase was immobilize d above the oxidase enzymes. To separate the two enzyme layers and to introduce an additional diffusion barrier a third pHEMA membrane witho ut enzyme was placed between the oxidase and catalase enzyme membranes . The intended use of this device is the measurement of undiluted biol ogical fluids in a clinical analyser. The sensors were tested in buffe r solutions and undiluted serum and exhibited excellent performances.