METHOD AND APPARATUS FOR THE DETECTION OF THE BINDING REACTION OF IMMUNOGLOBULINS

A method and apparatus for the detection of the presence of an analyte in fluid and the measurement of its concentration by means of a bioch emical binding system have been developed. The flow-injection-analysis cell has been constructed to detect analytes by amperometric or condu ctometric measurement. Several electrochemically active substances hav e been proposed as labels for the development of immunosensors: o-amin ophenol and derivatives, and polyaniline. The acceptability of differe nt kinds of electroactive substances, selectivity and sensitivity of s ensor response have been studied. It has been shown that the method se nsitivity is similar to that of ELISA; the response time is only sever al minutes.