Rc. Brady et Mr. Schleiss, IDENTIFICATION AND CHARACTERIZATION OF THE GUINEA-PIG CYTOMEGALOVIRUSGLYCOPROTEIN-H GENE, Archives of virology, 141(12), 1996, pp. 2409-2424
Subunit vaccines which target viral envelope glycoproteins offer promi
se for the prevention of congenital cytomegalovirus (CMV) infection. T
he guinea pig model of CMV infection is uniquely well suited to testin
g vaccines for prevention of congenital infection, since, in contrast
to other animal cytomegaloviruses, the guinea pig CMV (GPCMV) crosses
the placenta, producing intrauterine infection. Antibody to the CMV gl
ycoproteins B (gB) and H (gH) appears to be important in conferring pr
otective immunity. Unfortunately, little is known about specific GPCMV
envelope glycoproteins. Sequencing of GPCMV genome fragments was ther
efore undertaken to test whether GPCMV encodes a gH homologue. Partial
sequencing of the Hind III A fragment of the GPCMV genome revealed an
open reading frame of 2 169 nucleotides capable of encoding a protein
of 723 amino acids. Computer matrix analyses demonstrated identity be
tween this ORF and the gH coding sequences of other herpesviruses. The
GPCMV gH ORF encodes 12 highly conserved cysteine residues, contains
9 potential N-linked glycosylation sites, and has a predicted M(r) of
81.6 kDa. Northern blot hybridizations with gH-specific probes identif
ied an abundant 5.1 kb mRNA with expression kinetics of an ''early'' g
ene. A polyclonal antiserum raised against a synthetic peptide derived
from the deduced amino acid sequence of the gH ORF identified a virio
n-associated protein with an approximate M(r) of 85-kDa, the putative
GPCMV gH, in immunoblot assays.