Rb. Willis et Me. Montgomery, MEASUREMENT OF AMORPHOUS FERRIC PHOSPHATE AS AN ASSESSMENT OF IRON BIOAVAILABILITY, Analytical chemistry, 66(11), 1994, pp. 1832-1836
A method for measuring amorphous ferric phosphate in complex salt mixt
ures and animal diets is described. The procedure uses citrate solutio
ns for extraction of salt mixtures and tartrate solution for extractio
n of prepared diets. Tron in the solution is then determined colorimet
rically. Crystalline ferric phosphate, which has no iron bioavailabili
ty, is not extracted by either solution. Thus, the procedure can deter
mine if the amorphous form, which has a high iron bioavailability, is
present. The procedure was tested on gypsy moth artificial diet and We
sson salt mixture, which is a salt supplement of the diet.