PURIFICATION AND PARTIAL AMINO-ACID-SEQUENCE OF CURVATICIN FS47, A HEAT-STABLE BACTERIOCIN PRODUCED BY LACTOBACILLUS-CURVATUS FS47

Curvaticin FS47, a bacteriocin produced by Lactobacillus curvatus FS47 , is inhibitory to Listeria monocytogenes, as well as Lactobacillus, P ediococcus, Enterococcus, and Bacillus spp. The bacteriocin was purifi ed by 40% ammonium sulfate precipitation, solid-phase extraction, and reversed-phase high-pressure liquid chromatography. Purified curvatici n FS47 was determined to be 4.07 kDa by mass spectrometry and was part ially sequenced. Thirty-one N-terminal amino acids were identified; th e curvaticin FS47 protein sequence did not show homology to the pedioc in-like group of bacteriocins.