A. Kolman et al., IN-VITRO TRANSFORMATION OF C3H 10T1/2 AND SHE CELLS BY PROPYLENE-OXIDE AND EPICHLOROHYDRIN/, ATLA. Alternatives to laboratory animals, 22(3), 1994, pp. 180-184
Neoplastic cell transformation by the carcinogenic epoxides, propylene
oxide (PO) and epichlorohydrin (ECH), was investigated in two mammali
an assays involving murine C3H/10T1/2 cells and Syrian hamster embryo
(SHE) cells. In C3H/10T1/2 cells, PO increased the frequency of morpho
logical transformations 9-18 times compared to the background frequenc
y, at concentrations of 2.5-10mM (1-hour treatment). In SHE cells, a s
imilar increase (5-15 times) was reached at concentrations of 5-20mM (
8-day treatment). In both assays, ECH increased the frequency of morph
ological transformations only at the highest exposure levels (2mM for
C3H/10T1/2 cells and 0.5mM for SHE cells).