Vv. Chupin et al., H-2-NMR STUDY OF MEMBRANES COMPOSED OF DI ACYL, ALKYLACYL, ACYLALKYL AND DIALKYL PHOSPHATIDYLCHOLINES, Biologiceskie membrany, 11(1), 1994, pp. 89-97
A comparative study of model membranes formed from 1-12-H-2(2)] mitoyl
-rac-glycero-3-phospho-[gamma-H-2(3)]choline (H-2(5)-DPPC), 1-O-[2-H-2
(2)] mitoyl-rac-glycero-3-phospho-[gamma-H-2(3)]choline (H-2(5)-HPPC),
adecyl-rac-glycero-3-phospho-[gamma-H-2(3)]choline (H-2(5)-PHPC), and
mitoyl-rac-glycero-3-phospho-]gamma-H-2(3)]choline (H-2(5)-PHPC) in e
xcess of water has been performed by H-2-NMR. The dynamics and conform
ation of the labelled molecular segments have been estimated on the ba
sis of the spin-lattice relaxation time and residual quadrupolar split
ting. The mobility of the N-(CH3)-H-2 group has been shown to be the s
ame in all membranes studied in both liquid crystalline and gel state.
Quadrupolar splitting of N-(CH3)-H-2 groups increases in the order H-
2(5)-DPPC < H-2(5)-HPPC almost-equal-to H-2(5)-PHPC < H-2(5)-DHPC in g
el and liquid crystalline membranes. The orientation of the 2-(CH2)-H-
2 segment in liquid crystalline membranes is the same for all lipids i
nvestigated. The mobility of this fragment is lower in the membranes f
ormed from H-2(5)-DPPC and H-2(5)-PHPC.