MONITORING OF LATERAL ORGANIZATION OF PHO SPHOLIPIDS IN MEMBRANES WITH THE HELP OF LIPID-SPECIFIC FLUORESCENT-PROBES - BINARY-SYSTEMS PHOSPHATIDYLCHOLINE PHOSPHATIDYLETHANOLAMINE

A possibility to monitor the lateral organization of membranes compose d of DMPC - DMPE and DMPC - DPPE mixtures with the fluorescent lipid p robes bearing anthrylvinyl group in the hydrophobic region was studied . It has been found that the probes detect adequately the presence of lateral segregation in the DMPC - DMPE system but prefer the liquid ph ase in the DMPC - DPPE one. The ability of the phospholipid probes to record all stages of phase transition, and the pretransition of DMPC a s well has been shown. We can conclude that in the biological temperat ure range, anthrylvinyl-labeled lipids follow in a reliable mode the b ehavior of their natural analogs and permit to perform their different ial probing in heterogeneous membrane systems.