MOLECULAR-CLONING OF OZONE-INDUCIBLE PROTEIN FROM PINUS-SYLVESTRIS L WITH HIGH SEQUENCE SIMILARITY TO VERTEBRATE 3-HYDROXY-3-METHYLGLUTARYL-COA-SYNTHASE

Authors
WEGENER A GIMBEL W WERNER T HANI J ERNST D SANDERMANN H
Citation
A. Wegener et al., MOLECULAR-CLONING OF OZONE-INDUCIBLE PROTEIN FROM PINUS-SYLVESTRIS L WITH HIGH SEQUENCE SIMILARITY TO VERTEBRATE 3-HYDROXY-3-METHYLGLUTARYL-COA-SYNTHASE, Biochimica et biophysica acta, N. Gene structure and expression, 1350(3), 1997, pp. 247-252
Citations number
59
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1350
Issue
3
Year of publication
1997
Pages
247 - 252
Database
ISI
SICI code
0167-4781(1997)1350:3<247:MOOPFP>2.0.ZU;2-C
Abstract
We have isolated a Pinus sylvestris cDNA encoding a globular protein o f 474 amino acids with a predicted molecular weight of 52 995 Da. The deduced amino acid sequence showed 41.9% identity and 13.6% similarity to mammalian cytosolic 3-hydroxy-3-methylglutaryl-CoA-synthase (HMGS) . Treatment of Scots pine seedlings with ozone resulted in a transient increase of a 1.95 kb transcript, whereas a 1.2 kb mRNA decreased tra nsiently, indicating a possible influence of ozone on isoprenoid biosy nthesis.