PULMONARY FLAVIN-CONTAINING MONOOXYGENASE (FMO) IN RHESUS MACAQUE - EXPRESSION OF FMO2 PROTEIN, MESSENGER-RNA AND ANALYSIS OF THE CDNA

Authors
YUEH MF KRUEGER SK WILLIAMS DE
Citation
Mf. Yueh et al., PULMONARY FLAVIN-CONTAINING MONOOXYGENASE (FMO) IN RHESUS MACAQUE - EXPRESSION OF FMO2 PROTEIN, MESSENGER-RNA AND ANALYSIS OF THE CDNA, Biochimica et biophysica acta, N. Gene structure and expression, 1350(3), 1997, pp. 267-271
Citations number
22
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1350
Issue
3
Year of publication
1997
Pages
267 - 271
Database
ISI
SICI code
0167-4781(1997)1350:3<267:PFM(IR>2.0.ZU;2-R
Abstract
Pulmonary microsomes from Rhesus macaque express a flavin-containing m onooxygenase (FMO) resembling the FMO2 ortholog from rabbit with respe ct to immunochemical cross-reactivity and expression in lung, but not liver. A full-length cDNA was cloned following screening of a Rhesus m acaque lung cDNA library. The nucleotide sequence contained an open re ading frame encoding 535 amino acids with 85 and 84% identity to FMO2 from rabbit and guinea pig, respectively, and an identical location of the putative FAD- and NADP-binding sites. Northern blots of monkey lu ng mRNA revealed multiple size FMO2 transcripts. These mRNA transcript s are expressed in lung, but not in liver or kidney. (C) 1997 Elsevier Science B.V.