CHARACTERIZATION OF HUMAN CYCLOOXYGENASE-2 GENE PROMOTER LOCALIZATIONOF A TGF-BETA RESPONSE ELEMENT

Authors
YANG XH HOU FX TAYLOR L POLGAR P
Citation
Xh. Yang et al., CHARACTERIZATION OF HUMAN CYCLOOXYGENASE-2 GENE PROMOTER LOCALIZATIONOF A TGF-BETA RESPONSE ELEMENT, Biochimica et biophysica acta, N. Gene structure and expression, 1350(3), 1997, pp. 287-292
Citations number
26
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1350
Issue
3
Year of publication
1997
Pages
287 - 292
Database
ISI
SICI code
0167-4781(1997)1350:3<287:COHCGP>2.0.ZU;2-I
Abstract
We characterized 2.3 kb of 5'-flanking region of the human cyclooxygen ase 2 gene and analyzed its promoter activity. We localized three posi tive and two negative cis-acting regulatory regions and a TGF-beta res ponse region. Basal promoter activity was located between -77 and +99 bp. Maximal promoter activity was observed from -1838 to +99 bp. A TGF -beta response region was found between -454 and -288 bp. This region included an NF-I site. Electrophoretic mobility shift assays showed th at TGF-beta caused a marked change in the pattern and intensity of thi s NF-I like protein complex.