THE HUMAN MCP-3 GENE (SCYA7) - CLONING, SEQUENCE-ANALYSIS, AND ASSIGNMENT TO THE C-C CHEMOKINE GENE-CLUSTER ON CHROMOSOME 17Q11.2-Q12

Monocyte chemotactic proteins (MCPs) are chemokines involved in macrop hage recruitment during inflammation and cancer. A fall-size MCP-3 cDN A was used to isolate the functional human MCP-3 gene. Based on restri ction analysis, subclones were selected and the MCP-3 gene sequence wa s completed In addition to a dense region with direct and inverted rep eats and palindromic sequences, a double microsatellite (CA)(n)-(GA)(n ), was found at the 5'-end of the MCP-3 gene, and an RFLP was detected . The gene was regionally mapped by fluorescence in situ hybridization to human chromosome 17, subbands q11.2-q12. This site contains the MC P-subset of C-C chemokines and can be distinguished from the syntenic MIP-la locus. SCYA7 was assigned as the locus symbol of the MCP-3 gene . Double-labeling experiments confirmed the regional assignment of the MCP-3 gene close to the ERBB2 locus on human chromosome 17. (C) 1994 Academic Press, Inc