MAPPING OF THE GENES ENCODING HUMAN INDUCIBLE AND ENDOTHELIAL NITRIC-OXIDE SYNTHASE (NOS2 AND NOS3) TO THE PERICENTRIC REGION OF CHROMOSOME-17 AND TO CHROMOSOME-7, RESPECTIVELY

Nitric oxide (NO) is an important molecular messenger regulating the f unctions of a wide variety of cells and tissues. NO is synthesized fro m L-arginine by a variety of isoforms of the enzyme nitric oxide synth ase (NOS). We have used Southern blotting analysis on DNAs obtained fr om a panel of human-rodent hybrid cell lines to map the gene encoding the inducible NOS (NOS2) to chromosome 17cen-17q11 and the gene encodi ng the endothelial form of NOS (NOS3) to chromosome 7. Fluorescence in situ hybridization using a NOS2 probe gave several signals in the 17p 11-q11 pericentromeric region. (C) Academic Press, Inc.