M. Arendrup et al., HIV-1 INFECTION OF IN-VITRO CULTURED HUMAN MONOCYTES - EARLY EVENTS AND INFLUENCE OF ANTI HIV-1 ANTIBODIES, Archives of virology, 136(1-2), 1994, pp. 35-52
To characterize the role of the humoral immune response on HIV-1 infec
tion of monocytes and macrophages (M phi s) we examined the susceptibi
lity of in vitro cultured monocyte/M phi s to various HIV-1 isolates a
nd the influence of heterologous and particularly autologous anti HIV-
1 sera on this infection. Depending on the period of in vitro cultivat
ion and the virus isolate used different patterns of susceptibility we
re detected. One week old monocyte/M phi s were highly susceptible to
HIV-1 infection, in contrast to monocyte/M phi s cultured 4 weeks. The
infection by virus isolated immediately after seroconversion lead to
persistent infection with high level of antigen production in contrast
to infection by homologous virus isolated later. MAb against the V3-I
IIB loop and sCD4 inhibited the infection of monocyte/M phi s in a dos
e dependent manner, indicating that infection requires binding to CD4
and that post binding events may be common to the infection of lymphoc
ytes. Anti HIV-1 sera showed neutralizing activity against heterologou
s and even autologous escape virus. This finding, together with the ob
servation that monocytes and M phi s are infected in vivo, suggests th
at protection against HIV-1 infection of monocytes and M phi s in vivo
may not be obtainable by the humoral immune response alone.