HUMAN PERIPHERAL GAMMA-DELTA-T-CELLS ARE STIMULATED BY DAUDI BURKITTS-LYMPHOMA AND NOT BY ANY OTHER BURKITTS-LYMPHOMA TESTED

Daudi Burkitt's lymphoma cells have molecules on their surface which c an stimulate proliferation of human gamma delta T cells, while Raji, a nother Burkitt's lymphoma, cannot stimulate human gamma delta T cells. Human peripheral gamma delta T cells, coexpressing the V(gamma)9/(V d elta)2 chains of the T cell receptor, lyse Daudi cells but not Raji ce lls. Here, we have screened four other Burkitt's lymphoma cell lines ( HH514, DG75, Ramos, and Wilson), as well as cells derived from a fresh Burkitt's lymphoma, to see if any of them can be recognized by human gamma delta T cells. Leukemia-derived lines MOLT-4, CEM, and K562 have also been included in these studies. Among the Burkitt's lymphomas te sted, only Daudi, DG75, and HH514 could be lysed by V(gamma)9/V(delta) 2(+) T cell clones derived from the peripheral blood of healthy donors . These T cell clones were also able to lyse the NK sensitive leukemia lines K562 and MOLT-4. When bulk cultures of peripheral blood mononuc lear cells from healthy donors were cultured with different Burkitt's lymphoma or leukemia cell lines, only Daudi stimulated the outgrowth o f gamma delta T cells. Similarly, only Daudi cells could stimulate pro liferation of gamma delta T cell clones, and the response was enhanced significantly in the presence of interleukin-2. These data and our pr ior observations showing the use of the V(gamma)9/V(delta)2 TCR type b y Daudi-reactive human gamma delta T cells indicate that Daudi cells a re not representative of other Burkitt's lymphoma cell lines. (C) 1994 Academic Press, Inc.