Due to the importance of peptides and the absence of any-analytical me
thod for their study in wine using HPLC, an analytical method was deve
loped which includes ultrafiltration for the separation of proteins an
d low pressure column chromatography using Sephadex LH-20 for separati
on of free amino acids and phenolic compounds. Peptide derivatives wit
h o-phthalaldehyde were separated by reverse phase HPLC. Principal ami
no acids from peptides are: glutamic and aspartic acids, serine, glyci
ne, alanine, and lysine.