Wt. Willian et Tc. Mueller, LIQUID-CHROMATOGRAPHIC DETERMINATION OF NORFLURAZON AND ITS INITIAL METABOLITE IN SOIL, Journal of AOAC International, 77(3), 1994, pp. 752-755
A rapid, sensitive method for the determination of norflurazon in 4 so
ils is described. Data on the initial soil metabolite is also obtained
in soils with low organic matter. The method consists of extraction o
f soil samples with methanol, filtration, liquid chromatographic separ
ation of methanol-soluble components by using a C18 column, and fluore
scence detection with excitation at 294 nm and emission measured at 39
8 nm. Recoveries from fortified soils were >90% for norflurazon and >8
0% for desmethylnorflurazon from the Shipps, Lexington, and Harkey soi
ls. Average percent relative standard deviations over the soils examin
ed was 5.5% for norflurazon and 8.7% for desmethylnorflurazon. The lim
it of detection for norflurazon was 10 ng/g soil, whereas the limit of
detection for desmethylnorflurazon was 100 ng/g soil because of its s
maller relative detector response.