INFECTION OF HUMAN BONE-MARROW STROMAL CELLS BY HEPATITIS-B VIRUS - IMPLICATIONS FOR VIRAL PERSISTENCE AND THE SUPPRESSION OF HEMATOPOIESIS

Suspension cultures of bone marrow cells (BMC) were challenged with he patitis B virus (HBV) to study interactions between the virus and the nonadherent and adherent BMC populations. Virus-challenged BMC develop ed an adherent stromal layer that differed in cellular composition fro m that of mock-infected cultures, showing a threefold increase in cell s of the monocyte-macrophage lineage with an accompanying decrease in cells of the granulocytic lineage. Both viral envelope hepatitis B sur face and core antigen expression was detected in adherent and nonadher ent cell populations up to 10 days after virus challenge, which decrea sed thereafter. HBV DNA was still detectable in adherent cells 3 weeks after virus challenge, as shown by polymerase chain reaction analysis . These data indicate that HBV can infect not only bone marrow colony- forming cells but also the stromal cell populations involved with the regulation of hematopoiesis in vivo. Such virus-cell interactions coul d contribute to the immune dysfunction and bone marrow failure occasio nally reported for patients with HBV infection as well as acting as an important site for HBV latency and persistence.