GLU742 SUBSTITUTION TO LYS ENHANCES THE EDTA TOLERANCE OF ESCHERICHIA-COLI PQQ GLUCOSE-DEHYDROGENASE

Authors
SODE K SANO H
Citation
K. Sode et H. Sano, GLU742 SUBSTITUTION TO LYS ENHANCES THE EDTA TOLERANCE OF ESCHERICHIA-COLI PQQ GLUCOSE-DEHYDROGENASE, Biotechnology letters, 16(5), 1994, pp. 455-460
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
16
Issue
5
Year of publication
1994
Pages
455 - 460
Database
ISI
SICI code
0141-5492(1994)16:5<455:GSTLET>2.0.ZU;2-Y
Abstract
Based on homology analysis of the PQQ (pyrroloquinoline quinone) gluco se dehydrogenase (PQQGDH) gene from Esherichia coli and Acinetobacter calcoaceticus, Glu742 was substituted to Lys by site directed mutagene sis of the E. coli PQQGDH gene (gcd). The mutant enzyme, E742K showed higher tolerance towards EDTA inactivation than wild type PQQGDH. This is the first mutagenesis study of putative a PQQ binding site in PQQ enzyme.