Pseudomonas sp. IMD 353 produced an extracellular consititutive maltot
etraose-forming amylase in a medium containing glucose (or fructose),
yeatex and mineral salts. Km values an starch, amylopectin and short c
hain amylose were 4.0, 2.8 and 3.0 mg/ml, respectively. Sulphydryl red
ucing agents activated the enzyme considerably, as did Co2+ and Mn2+.