ELASTOLYTIC ACTIVITY FROM FLAVOBACTERIUM-ODORATUM - MICROBIAL SCREENING AND CULTIVATION, ENZYME-PRODUCTION AND PURIFICATION

Authors
YAN ZY GUAN GX ZHANG WQ LIN ZF
Citation
Zy. Yan et al., ELASTOLYTIC ACTIVITY FROM FLAVOBACTERIUM-ODORATUM - MICROBIAL SCREENING AND CULTIVATION, ENZYME-PRODUCTION AND PURIFICATION, Process biochemistry, 29(6), 1994, pp. 427-436
Citations number
16
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Process biochemistry → ACNP
ISSN journal
13595113
Volume
29
Issue
6
Year of publication
1994
Pages
427 - 436
Database
ISI
SICI code
1359-5113(1994)29:6<427:EAFF-M>2.0.ZU;2-Z
Abstract
Flavobacterium odoratum, a strain of elastolytic enzyme producing bact erium was isolated from soil. It can be cultivated from a medium conta ining poultry feather metal as a main nitrogen source producing 350 un its per ml of cultural broth of extracellular elastolytic enzyme. The purified elastase has a molecular weight of 21 300, and formed long, f ine needle-shaped crystals. It showed an optimal pH at 7.4, optimum te mperature at 50-degrees-C and was stable under 30-degrees-C and in the range between pH 5 and pH 10. The digestibility of some protein subst rates by the enzyme was observed