ADSORPTION AND BINDING OF THE INSECTICIDAL PROTEINS FROM BACILLUS-THURINGIENSIS SUBSP KURSTAKI AND SUBSP TENEBRIONIS ON CLAY-MINERALS

The equilibrium adsorption and binding of the toxins from Bacillus thu ringiensis subsp. kurstaki (Btk) (66 kDa), toxic to lepidopteran larva e, and from subsp. tenebrionis (Btt) (68 kDa), toxic to coleopteran la rvae, on the clay minerals, montmorillonite (M) and kaolinite (K), hom oionic to various cations ('clean' clays) or coated with two types of polymeric oxyhydroxides of Fe(III) ('dirty' clays) were studied. Adsor ption of the toxins on a constant amount of the clays increased with t oxin concentration and then reached a plateau. Larger amounts of the t oxins from Btk than from Btt were adsorbed. Adsorption of the toxins w as rapid (< 30 min for maximal adsorption of the toxins from Btk; < 30 min for 70% of maximal adsorption of the toxins from Btt, which was c omplete at 3 h), and maximal between pH 6 and 8 onto clean clays and b etween pH 5 and 9 onto dirty clays. Adsorption of the toxins from Btk or Btt on clean clays was affected by the type of cation to which the clays were homoionic. The adsorption of the toxins from Btk was greate r on M homoionic to monovalent than to polyvalent cations, and adsorpt ion decreased as the valency of the charge-compensating cation increas ed, with the exception of M homoionic to La, which adsorbed more than M homoionic to divalent cations or to Al. The amounts of toxins from B tt adsorbed were also greater on M homoionic to monovalent than to di- and trivalent cations, with the exception of M homoionic to Mg, which adsorbed the most. Adsorption of the toxins from both Btt and Btk on K was significantly lower than on M, and the valency of the charge-com pensating cations on K had little effect on adsorption. Smaller amount s of the toxins from Btk and Btt were adsorbed on dirty clays than on clean clays. Only ca 10 and 30% of the toxins from Btk and Btt, respec tively, adsorbed at equilibrium were desorbed by one or two washes wit h water. Additional washings desorbed no more toxins, indicating that the toxins were tightly bound on the clays. The formation of complexes between the toxins and the clays did not appear to alter significantl y the structure of the toxins, as indicated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) of the equilibrium super natants and desorption washes and by dot-blot enzyme-linked immunosorb ent assays and Fourier-transform infrared analyses of the bound toxins . The toxins partially intercalated M, with more intercalation by the toxins from Btt. However, the entire proteins did not appear to penetr ate M. There was no intercalation of K.