CHANGES IN POLYPEPTIDE PATTERNS IN TOBACCO ROOTS COLONIZED BY 2 GLOMUS SPECIES

Changes in gene expression were studied during the establishment of ar buscular mycorrhizal symbiosis in tobacco roots from an amphidiploid h ybrid Nicotiana glutinosa x N. debneyi. Polypeptide patterns from cont rol roots and from roots infected by Glomus mosseae or G. intraradices were resolved by two-dimensional polyacrylamide gel electrophoresis a nd followed in a time-course analysis. Arbuscular mycorrhizal infectio n led to significant modifications in polypeptide patterns with: (a) d ecreased amounts of some polypeptides, (b) increased accumulation of o thers, and (c) appearance of newly-induced polypeptides. Comparisons m ade during infection development by the two Glomus species demonstrate d that protein modifications changed in relation to the mycorrhizal st ate of the tobacco roots.