J. Naus et al., MODEL STUDIES OF CHLOROPHYLL FLUORESCENCE REABSORPTION AT CHLOROPLASTLEVEL UNDER DIFFERENT EXCITING CONDITIONS, Photosynthesis research, 40(1), 1994, pp. 67-74
A theoretical model is presented describing the distortion of chloroph
yll fluorescence spectra of a chloroplast or a group of chloroplasts b
y the effect of fluorescence reabsorption. Model calculations using th
e experimental data show that the primary reabsorption effect occurs a
lready within one chloroplast and the spectral distortion depends sign
ificantly on the excitation regime of the chloroplast. A theoretical d
ependence of the distortion function, defined as a change in the F(685
)/F(735) fluorescence band ratio, on the mean chlorophyll concentratio
n in a chloroplast is predicted for different light excitation regimes
. The distortion of measured chlorophyll fluorescence spectra at 77 K
of chloroplast suspension adsorbed on filter papers of two strongly di
fferent diffusive reflectivities and at different mean chlorophyll con
centrations are discussed with the help of the presented theory.