Rr. Dubreuil et al., MONOCLONAL-ANTIBODIES DIRECTED AGAINST HUMAN TUMOR-ASSOCIATED ANTIGENS CROSS-REACT WITH DROSOPHILA PROTEINS IN CLUSTERS, International journal of cancer, 1994, pp. 96-97
We reacted the Third International IASLC Workshop panel of monoclonal
antibodies (MAbs) directed against human lung-tumor-associated epitope
s with nitrocellulose blots of total proteins from adult fruit flies.
Out of 63 MAbs tested, 9 showed a significant reaction with Drosophila
proteins. Interestingly, in a double-blind analysis, most of the posi
tive reactions fell into clusters that parallel the antibody reactivit
ies against human tissues. In light of our findings, it becomes possib
le to screen expression vector libraries in order to isolate Drosophil
a cDNA that may have homology with human tumor-associated antigens. (C
) 1994 Wiley-Liss, Inc.