A FOOD-GRADE SILICON DIOXIDE IS HYPOCHOLESTEROLEMIC IN THE DIET OF CHOLESTEROL-FED RATS

Silicon dioxide, commonly referred to as silica, is present in plant c ell walls and interstitial spaces, and is often found as a component o f dietary fibers that have exhibited hypocholesterolemic activity in a nimals. The primary objective of this study was to determine the chole sterolemic effects of two different morphological forms of silicon in the diet of cholesterol-fed rats. Male Wistar rats were provided diets containing 1 g cholesterol/100 g diet, and 0.65 g Si/100 g diet as ei ther a sodium salt (silicate group) or silicon dioxide, a synthetic si lica polymer (silica group). Cellulose was used as a control (control group). The in vitro bile acid binding capacity of the SiO2 was also m easured. After 44 d of diet administration, animals were deprived of f ood for 24 h and then killed. Plasma total, VLDL, and LDL cholesterol concentrations were 18%, 29%, and 26% lower, respectively, in the sili ca group than in the control group. However, liver cholesterol concent rations were not different among dietary treatments. During the initia l 15 d of the study, average daily total fecal bile acids were 38% hig her in the silica group than in the control group, but fecal bile acid outputs were not different for the remainder of the experiment. The s ilica polymer used in the feeding trial was found to adsorb 5 times mo re cholate than chenodeoxycholate, at pH 7.5 in vitro. In vivo, the po tential for silica to enhance fecal cholic acid excretion, relative to chenodeoxycholic acid during the initial stage of the study, may have contributed to the hypocholesterolemic response to the silica diet. I t is further hypothesized that normalization of fecal bile acid levels in the silica group may have been due to an adaptation response that involved changes in the spectrum of bile acids in the enterohepatic po ol.