EXPRESSION OF THE CELLULASE (FI-CMCASE) GENE OF ASPERGILLUS-ACULEATUSIN SACCHAROMYCES-CEREVISIAE

As a step to breed a Saccharomyces cerevisiae strain able to produce e thanol directly from cellulose, we combined cDNA for Aspergillus acule atus FI-CMCase (FI-carboxymethyl cellulase) with the GAP (glyceraldehy de-3-phosphate dehydrogenase) promoter of S. cerevisiae and used the r esultant plasmid, pYEC91, to transform S. cerevisiae. The transformed cells produced active FI-CMCase within the cytoplasm. Western-blot ana lysis following SDS-polyacrylamide gel electrophoresis demonstrated th at the cells contained a peptide having the same molecular mass and im munological identity as A. aculeatus FI-CMCase.