TRANSFORMATION OF ACETOBACTER POLYOXOGENES WITH PLASMID DNA BY ELECTROPORATION

Acetobacter polyoxogenes was transformed with plasmid DNA by electropo ration. The following points were essential for transformation: (i) di lution of the culture broth with cold water and air bubbling of the cu lture broth for transformation at discharging from a jar fermenter, an d (ii) selection of transformants by liquid cultivation. For shortenin g of the lag time in cultivation for selection of transformants, the f ollowing treatments were useful: (i) addition of sucrose to the cell s uspension during transformation and to the broth for cultivation, and (ii) addition of 1 mM MgCl2 to a mixture of cells and DNA during elect roporation.