INTRODUCING BT GENE INTO MAIZE WITH OVARY INJECTION

It is reported here that Bt toxin gene has been successfully transferr ed into maize inbred line by ovary injection for the first time both a t home and abroad. One transgenic plant (To) has been confirmed by Sou thern blotting and PCR test, and 71 progenies (T1) from T0 have been o btained through self-pollination. Of these 71 progenies, seven plants demonstrated positive results in the PCR test; four were used to feed Asian corn borer, and certain effect of insect-resistance was observed . The experiments on the ovary injection in Hainan Province have also been repeated, thus providing new chance to the application of genetic engineering to the maize improvement.