SYNTHESIS OF AN ACETYLCHOLINE RECEPTOR-SPECIFIC TOXIN DERIVATIVE REGIOSELECTIVELY LABELED WITH AN UNDECAGOLD CLUSTER

Authors
KESSLER P KOTZYBAHIBERT F LEONETTI M BOUET F RINGLER P BRISSON A MENEZ A GOELDNER MP HIRTH C
Citation
P. Kessler et al., SYNTHESIS OF AN ACETYLCHOLINE RECEPTOR-SPECIFIC TOXIN DERIVATIVE REGIOSELECTIVELY LABELED WITH AN UNDECAGOLD CLUSTER, Bioconjugate chemistry, 5(3), 1994, pp. 199-204
Citations number
35
Categorie Soggetti
Biology,Chemistry
Journal title
Bioconjugate chemistry → ACNP
ISSN journal
10431802
Volume
5
Issue
3
Year of publication
1994
Pages
199 - 204
Database
ISI
SICI code
1043-1802(1994)5:3<199:SOAART>2.0.ZU;2-9
Abstract
The regioselective modification of a snake curaremimetic toxin is desc ribed. Toxin-alpha from Naja nigricollis was derivatized with an elect ron-dense maleimido undecagold cluster 5. The cluster-bound obtained t oxin 8 has a very high affinity for the cholinergic binding site (K(i) = 70 pM) of Torpedo marmorata nicotinic receptor. It is harboring a c ompact heavy atom core of about 8 angstrom which makes it very useful for electron microscopy experiments.