SYNTHESIS OF EUROPIUM(III) CHELATES SUITABLE FOR LABELING OF BIOACTIVE MOLECULES

Two different kinds of europium(III) chelates, luminescent and nonlumi nescent, were prepared. The chelates were coupled to bioanalytical rea gents, such as antibodies, after activations of the amino group on the chelates with thiophosgene, 2,4,6-trichloro-1,3,5-triazine, or iodoac etic anhydride. The reactivities of the activated luminescent chelates in the labeling of antibodies as well as the effects of both the coup ling ratio and the linkage group to the luminescence quantum yield of the antibody-bound chelate were studied in aqueous buffer solution.