SOLID-PHASE EXTRACTION APPLIED TO THE DETERMINATION OF BIOGENIC-AMINES IN WINES BY HPLC

A method suitable for the determination of 19 biogenic amines in wine has been developed. The method involves derivatization of amines by tr eatment with dansyl chloride and solid-phase extraction of the derivat ives. Prior to the derivatization procedure, clean-up of the wine samp le with polyvinylpyrrolidone is carried out. Reversed-phase gradient e lution HPLC with UV detection at 250 nm was used to determine these co mpounds. Some consideration was given to the effect of temperature on the separation process. Linearity of derivatization was obtained for a mounts of all the biogenic amines ranging from 500 mu g.L(-1) to 20 mg .L(-1). Limits of detection (signal-to-noise ratio = 3) of the amines were similar for all the dansyl-derivatives (between 50 and 150 mu g.L (-1)). Addition of standard amines was used for the determination of a mine recoveries. These were better than 85 % for ethanolamine, tryptam ine, phenetylamine, putrescine, cadaverine and histamine. The overall process was succesfully applied to identify and quantify biogenic amin es in white and red wines.