C. Elfakir et al., RETENTION OF SOME GLUCOSINOLATES IN ANION-EXCHANGE CHROMATOGRAPHY .2.QUALITATIVE STUDY ON A STYRENE-DIVINYLBENZENE COPOLYMERIC ANION-EXCHANGER, Chromatographia, 38(9-10), 1994, pp. 585-590
The retention and separation of glucosinolates, as organic anions, wer
e studied on a styrene-divinyibenzene copolymeric anion exchanger unde
r isocratic elution conditions. The effects of the nature and concentr
ation of different inorganic and organic eluent anions, and the influe
nce of acetonitrile addition to the aqueous buffered mobile phase are
investigated. For the alkanesulfonate eluent anions, elution strength
increases noticeably with alkyl chain length. The plots of capacity fa
ctors of glucosinolates versus the reciprocal of organic eluent ion co
ncentration show good linearity. The values of the slope and y-interce
pt data confirm the importance of the reverse phase interactions with
respect to the ion exchange interactions in the glucosinolate retentio
n mechanism. Our results prove that the isocratic analysis of intact g
lucosinolates on a polymeric anion exchanger is a suitable and conveni
ent method that should be used as a complement to the current ion pair
ing chromatographic method.