RETENTION OF SOME GLUCOSINOLATES IN ANION-EXCHANGE CHROMATOGRAPHY .2.QUALITATIVE STUDY ON A STYRENE-DIVINYLBENZENE COPOLYMERIC ANION-EXCHANGER

The retention and separation of glucosinolates, as organic anions, wer e studied on a styrene-divinyibenzene copolymeric anion exchanger unde r isocratic elution conditions. The effects of the nature and concentr ation of different inorganic and organic eluent anions, and the influe nce of acetonitrile addition to the aqueous buffered mobile phase are investigated. For the alkanesulfonate eluent anions, elution strength increases noticeably with alkyl chain length. The plots of capacity fa ctors of glucosinolates versus the reciprocal of organic eluent ion co ncentration show good linearity. The values of the slope and y-interce pt data confirm the importance of the reverse phase interactions with respect to the ion exchange interactions in the glucosinolate retentio n mechanism. Our results prove that the isocratic analysis of intact g lucosinolates on a polymeric anion exchanger is a suitable and conveni ent method that should be used as a complement to the current ion pair ing chromatographic method.