LINKAGE GROUPS OF ISOZYMES, RFLP AND RAPD MARKERS IN CARROT (DAUCUS-CAROTA L SATIVUS)

Genetic and linkage analysis of marker loci were performed with 4 self ed progenies, derived from single plant (I-0/1 lines) of carrot (Daucu s carota L. sativus). The analysis of 58 markers included 1 morphologi cal marker, 10 isozyme loci, 14 RFLPs, 28 RAPD markers, and 6 isolated PCR fragments used as RFLP probes. Linkage analysis was carried out w ith the MAPMAKER program and resulted in the construction of 8 linkage groups containing 55 markers with an average distance of 13.1 cM, 3 m arker loci remained unlinked. 24% of the markers deviated significantl y from the expected Mendelian ratios (1:2:1 or 3:1)due to gametic or z ygotic selection. It was shown that isolated PCR amplification product s can be used as RFLP probes to detect polymorphisms for a certain loc us in progenies where the corresponding RAPD pattern is monomorphic or no amplification product is observed. Since carrot has a relative sma ll genome the probability of amplifying repetitive DNA sequences is co mparatively low. Thus PCR amplification products represent an addition al useful source of RFLP probes.