HISTONE H4 PROMOTER FOR EXPRESSION OF A PHLEOMYCIN-RESISTANCE GENE INPHANEROCHAETE-CHRYSOSPORIUM

In this study, two transformation vectors (pMG101 and pMG103) for Phan erochaete chrysosporium were constructed, based on the ble phleomycin- resistance-encoding gene and a homologous histone H4 promoter. Transfo rmation frequencies were 6-10 per mu g of DNA. Transformed vector DNA could either exist as an unstable replicating plasmid or could be stab ly integrated. Integrated vector DNA from pMG101, which also contains a histone-encoding H3 gene in the promoter fragment, becomes methylate d, resulting in inactivation of ble-dependent resistance. Plasmid pMG1 03, which lacks the H3, does not show methylation.