M. Gessner et U. Raeder, HISTONE H4 PROMOTER FOR EXPRESSION OF A PHLEOMYCIN-RESISTANCE GENE INPHANEROCHAETE-CHRYSOSPORIUM, Gene, 142(2), 1994, pp. 237-241
In this study, two transformation vectors (pMG101 and pMG103) for Phan
erochaete chrysosporium were constructed, based on the ble phleomycin-
resistance-encoding gene and a homologous histone H4 promoter. Transfo
rmation frequencies were 6-10 per mu g of DNA. Transformed vector DNA
could either exist as an unstable replicating plasmid or could be stab
ly integrated. Integrated vector DNA from pMG101, which also contains
a histone-encoding H3 gene in the promoter fragment, becomes methylate
d, resulting in inactivation of ble-dependent resistance. Plasmid pMG1
03, which lacks the H3, does not show methylation.