Ninety Chinese rice landraces were examined with special reference to
the indica-japonica differentiation in terms of traditional criteria,
isozyme analysis and PCR analysis of the chloroplast DNA (cpDNA). Cult
ivars were separated into indica and japonica defined by a discriminan
t function (Z) based on key characters, as well as by isozyme genotype
s. Most indica landraces had chloroplast DNAs with a deletion at the P
st-12 fragment, while most japonica landraces had cpDNAs without the d
eletion. Two traditionally recognized varietal groups in China, keng a
nd hsien, corresponded largely to the respective japonica and indica r
evealed in our study. The results obtained in this study showed good a
greement for classification of indica and japonica types by the three
methods: discriminant analysis by Z value, isozyme analysis, and PCR a
nalysis for cpDNA.