AN INSULIN WITH THE NATIVE SEQUENCE BUT VIRTUALLY NO ACTIVITY

The B24-B25 peptide bond of insulin was replaced by an ester bond. To our knowledge this is the first replacement of a main chain atom repor ted for the hormone. It is meant to eliminate a structurally important H-bond between the imino group of B25 and the carbonyl oxygen of A19, and consequently to enhance detachment of the C-terminal B chain from the underlying A chain. On the basis of independent experimental evid ence this very conformational change is believed to be a prerequisite for receptor binding. It was thus anticipated that increased flexibili ty would increase receptor binding and activity. Intriguingly, porcine [B24-B25 CO-O]insulin (depsi-insulin) and likewise [B24-B25 CO-O]des- (B26-B30)insulin-B25-amide (depsi-DPI-amide) were found to be only 3-4 % potent.