ISOLATION AND CHARACTERIZATION OF THE DROSOPHILA-MELANOGASTER EIF-2-ALPHA GENE ENCODING THE ALPHA-SUBUNIT OF TRANSLATION INITIATION-FACTOR EIF-2

Authors
QU SM CAVENER DR
Citation
Sm. Qu et Dr. Cavener, ISOLATION AND CHARACTERIZATION OF THE DROSOPHILA-MELANOGASTER EIF-2-ALPHA GENE ENCODING THE ALPHA-SUBUNIT OF TRANSLATION INITIATION-FACTOR EIF-2, Gene, 140(2), 1994, pp. 239-242
Citations number
16
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
140
Issue
2
Year of publication
1994
Pages
239 - 242
Database
ISI
SICI code
0378-1119(1994)140:2<239:IACOTD>2.0.ZU;2-L
Abstract
Genomic and cDNA clones encoding the Drosophila melanogaster alpha-sub unit of translational initiation factor 2 (elF-2alpha) were isolated. The D. melanogaster eIF-2alpha gene encodes a 341 amino-acid (aa) prot ein that shares 57 and 44% identity to its human and yeast homologues, respectively. The regulatory phosphorylation site at Ser50 is embedde d in a segment of 19 conserved aa residues. Analysis of the genomic DN A and cDNA clones indicated that eIF-2alpha is a single-copy gene and its coding region is interrupted by a 260-bp intron. The D. melanogast er eIF-2alpha mRNA is 1350 nt in length and is expressed throughout de velopment.