CLONING AND ACTIVATION OF THE SYRIAN-HAMSTER NEU PROTOONCOGENE

Authors
NAKAMURA T USHIJIMA T ISHIZAKA Y NAGAO M ARAI M YAMAZAKI Y ISHIKAWA T
Citation
T. Nakamura et al., CLONING AND ACTIVATION OF THE SYRIAN-HAMSTER NEU PROTOONCOGENE, Gene, 140(2), 1994, pp. 251-255
Citations number
17
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
140
Issue
2
Year of publication
1994
Pages
251 - 255
Database
ISI
SICI code
0378-1119(1994)140:2<251:CAAOTS>2.0.ZU;2-N
Abstract
Point mutations of the Syrian hamster neu proto-oncogene have been obs erved in the transmembrane domain of N-nitroso-N-ethylurea(ENU)-induce d neurofibromas. Genomic DNA derived from tumor tissue showed transfor ming activity in an NIH 3T3 assay and a cDNA clone of the hamster neu gene, containing the entire protein-coding region, was isolated from a transformant cDNA library. The encoded product is 92 and 88% homologo us to the rat neu and the human c-erbB-2. respectively. The product of the mutated hamster neu gene showed increased autophosphorylation of Tyr residues.