RELATIONSHIP OF CD34(-BLOOD STEM-CELL TRANSPLANTATION() CELL DOSE TO EARLY AND LATE HEMATOPOIESIS FOLLOWING AUTOLOGOUS PERIPHERAL)

We evaluated early and late hematopoietic reconstitution in 27 patient s with advanced lymphoma, Hodgkin's disease, and breast or ovarian can cer after treatment using high-dose/myeloablative conditioning regimen s and autologous peripheral blood stem cell (PBSC) transplantation. Ei ghteen patients (67%) received G-CSF 5 mu g/kg/day following chemother apy and nine (33%) were mobilized using G-CSF alone, Each patient had 7 x 10(8) mononuclear cells (MNC) per kg collected, G-CSF was administ ered post-PBSC infusion, While all patients showed prompt granulocyte recovery by day 14, platelet recovery failed to occur in four (15%) he avily pretreated patients with non-Hodgkin's lymphoma, Retrospective a nalysis in 17 patients revealed that the infused number of CD34 surfac e antigen-positive cells correlated with time to granulocyte (r = 0.59 , P = 0.012) and platelet (r = 0.58, P = 0.021) recovery, Patients rec eiving the higher numbers of CD34(+) cells had consistently better hem atologic parameters at all times examined, At 180 days post-transplant , the median Hb level was 124 g/l vs 88 g/l (P = 0.004); platelet coun t was 202 x 10(9)/l vs 25 x 10(9)/l (P = 0.004); and neutrophil count was 3100 x 10(6)/l vs 1400 x 10(6)/l (P = 0.15), Hemoglobin strongly c orrelated with the CD34(+) cell dose at 360 days (r = 0.90, P = 0.01), We conclude that graft CD34(+) cell content appears to be an indicato r of the quality of late as well as early hematopoietic function.