THE NUSG GENE OF STREPTOMYCES-GRISEUS - CLONING OF THE GENE AND ANALYSIS OF THE A-FACTOR BINDING-PROPERTIES OF THE GENE-PRODUCT

Authors
KUBERSKI S KASBERG T DISTLER J
Citation
S. Kuberski et al., THE NUSG GENE OF STREPTOMYCES-GRISEUS - CLONING OF THE GENE AND ANALYSIS OF THE A-FACTOR BINDING-PROPERTIES OF THE GENE-PRODUCT, FEMS microbiology letters, 119(1-2), 1994, pp. 33-39
Citations number
27
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
119
Issue
1-2
Year of publication
1994
Pages
33 - 39
Database
ISI
SICI code
0378-1097(1994)119:1-2<33:TNGOS->2.0.ZU;2-6
Abstract
The nusG gene of Streptomyces griseus was cloned and the nucleotide se quence determined. It encodes a protein with an identity of 76% to the reported receptor (VbrA) for VB-C, an autoregulatory factor in Strept omyces virginae. NusG protein was expressed in Escherichia coli. Howev er, no binding activity for A-factor, an butyrolactone autoregulator i n S. griseus very similar to VB-C, could be detected. The nusG gene of S. griseus does not seem to encode the A-factor-binding protein.