AN IMPROVED METHOD FOR DETECTING CYTOSTATIC TOXIN (EMETIC TOXIN) OF BACILLUS-CEREUS AND ITS APPLICATION TO FOOD SAMPLES

Authors
MIKAMI T HORIKAWA T MURAKAMI T MATSUMOTO T YAMAKAWA A MURAYAMA S KATAGIRI S SHINAGAWA K SUZUKI M
Citation
T. Mikami et al., AN IMPROVED METHOD FOR DETECTING CYTOSTATIC TOXIN (EMETIC TOXIN) OF BACILLUS-CEREUS AND ITS APPLICATION TO FOOD SAMPLES, FEMS microbiology letters, 119(1-2), 1994, pp. 53-57
Citations number
10
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
119
Issue
1-2
Year of publication
1994
Pages
53 - 57
Database
ISI
SICI code
0378-1097(1994)119:1-2<53:AIMFDC>2.0.ZU;2-V
Abstract
We developed an improved HEp-2 cell assay method for the detection of Bacillus cereus toxin, which affects the proliferation of HEp-2 cells. The cytostatic toxin was stable upon exposure to heat, pH 2, pH 11 an d trypsin, which suggests it is an emetic. Using the HEp-2 cell assay, we examined the distribution and contamination of B. cereus strains t hat produced an emetic toxin in various foods. Although there were 228 enterotoxin producers among 310 B. cereus strains obtained from foods , 16 of them produced the cytostatic type (emetic toxin). All of the s trains that produced the cytostatic toxin were of the H.1 serotype.