BIOCHEMICAL AND GENETIC-HETEROGENEITY OF STAPHYLOCOCCAL PROTEIN-A

Authors
SAKURADA J LI ZJ SEKI K MURAI M USUI A MORIHIGASHI M JITSUKAWA H SEONG HK MUTOU M MASUDA S
Citation
J. Sakurada et al., BIOCHEMICAL AND GENETIC-HETEROGENEITY OF STAPHYLOCOCCAL PROTEIN-A, FEMS microbiology letters, 119(1-2), 1994, pp. 59-63
Citations number
11
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
119
Issue
1-2
Year of publication
1994
Pages
59 - 63
Database
ISI
SICI code
0378-1097(1994)119:1-2<59:BAGOSP>2.0.ZU;2-A
Abstract
We investigated the biochemical and genetic heterogeneity of protein A from Staphylococcus aureus. SpA genes (spas) of various strains were heterogeneous when detected as DraI and EcoRV fragments of chromosomal DNA. Polymerase chain reaction using primers to detect DNA encoding t he IgG-binding domains in spa revealed that they numbered between 2 an d 5. Protein A from several S. aureus strains showed two types of reac tivities to immunoglobulins in normal canine serum according to the nu mber of active domains.