INFLUENCE OF UREMIA ON THE DETERMINATION OF BLOOD GLYCOHEMOGLOBIN BY HPLC, ELECTROPHORESIS AND AFFINITY-CHROMATOGRAPHY IN DIABETIC AND NONDIABETIC PATIENTS

Authors
HERRUER MH VANKOOTEN EAW SLUITER HE ZUIJDERHOUDT FMJ
Citation
Mh. Herruer et al., INFLUENCE OF UREMIA ON THE DETERMINATION OF BLOOD GLYCOHEMOGLOBIN BY HPLC, ELECTROPHORESIS AND AFFINITY-CHROMATOGRAPHY IN DIABETIC AND NONDIABETIC PATIENTS, European journal of clinical chemistry and clinical biochemistry, 32(5), 1994, pp. 361-364
Citations number
9
Categorie Soggetti
Biology,"Chemistry Medicinal
Journal title
European journal of clinical chemistry and clinical biochemistry → ACNP
ISSN journal
09394974
Volume
32
Issue
5
Year of publication
1994
Pages
361 - 364
Database
ISI
SICI code
0939-4974(1994)32:5<361:IOUOTD>2.0.ZU;2-M
Abstract
Glycohaemoglobin in human blood can be determined by several methods. We investigated three methods used in daily practice, viz. affinity ch romatography, HPLC and electrophoresis. We examined the influence of u raemia in diabetic and non-diabetic patients on the results obtained w ith HPLC and electrophoresis and compared these with the results obtai ned with affinity chromatography. Our current method (electrophoresis) proved to be very sensitive to uraemia giving falsely elevated levels of HbA1. HPLC was not disturbed by uraemia.