INTERPHASE MOLECULAR CYTOGENETICS OF EWINGS-SARCOMA AND PERIPHERAL NEUROEPITHELIOMA T(1122) WITH FLANKING AND OVERLAPPING COSMID PROBES

Authors
DESMAZE C ZUCMAN J DELATTRE O MELOT T THOMAS G AURIAS A
Citation
C. Desmaze et al., INTERPHASE MOLECULAR CYTOGENETICS OF EWINGS-SARCOMA AND PERIPHERAL NEUROEPITHELIOMA T(1122) WITH FLANKING AND OVERLAPPING COSMID PROBES, Cancer genetics and cytogenetics, 74(1), 1994, pp. 13-18
Citations number
14
Categorie Soggetti
Oncology,"Genetics & Heredity
Journal title
Cancer genetics and cytogenetics → ACNP
ISSN journal
01654608
Volume
74
Issue
1
Year of publication
1994
Pages
13 - 18
Database
ISI
SICI code
0165-4608(1994)74:1<13:IMCOEA>2.0.ZU;2-6
Abstract
The translocation, t(11;22)(q24;q12), recurrently observed in Ewing's sarcoma and in peripheral neuroepithelioma has been recently cloned. T he analysis of a series of ES/PNE has revealed that the chromosome 22 breakpoints are clustered in a small region of 7 kb, called EWSR1, and that those on chromosome 11 are spread over a larger region of 40-50 kb, called EWSR2. Cosmids from loci flanking or overlapping these two regions have been obtained. We demonstrate here that fluorescence in s itu hybridization (FISH) with these cosmids allows the localization of the two breakpoints with a 10-kb resolution and leads to a rapid and reliable ES/PNE diagnosis.