C. Desmaze et al., INTERPHASE MOLECULAR CYTOGENETICS OF EWINGS-SARCOMA AND PERIPHERAL NEUROEPITHELIOMA T(1122) WITH FLANKING AND OVERLAPPING COSMID PROBES, Cancer genetics and cytogenetics, 74(1), 1994, pp. 13-18
The translocation, t(11;22)(q24;q12), recurrently observed in Ewing's
sarcoma and in peripheral neuroepithelioma has been recently cloned. T
he analysis of a series of ES/PNE has revealed that the chromosome 22
breakpoints are clustered in a small region of 7 kb, called EWSR1, and
that those on chromosome 11 are spread over a larger region of 40-50
kb, called EWSR2. Cosmids from loci flanking or overlapping these two
regions have been obtained. We demonstrate here that fluorescence in s
itu hybridization (FISH) with these cosmids allows the localization of
the two breakpoints with a 10-kb resolution and leads to a rapid and
reliable ES/PNE diagnosis.