MIXED-MODE FLUORESCENT DNA-SEQUENCING

Fluorescence-based, automated DNA sequencers represent on of the major advances in recent molecular biology. Two main technologies have been developed in this field: the single-label/four-lane system and the fo ur-label/one-lane system. The following presents the use of single-lab el-sequencing chemistry, which resembles traditional radioactive DNA s equencing, using the four-label system ABI 373A that expands its flexi bility and obtains data that are immediately interpretable without sof tware manipulation. This method has been named mixed-mode fluorescent DNA sequencing. Here we show one of its possible applications in molec ular genetic analysis.