VIDEO IMAGING OF ETHIDIUM BROMIDE-STAINED DNA GELS WITH SURFACE UV ILLUMINATION

We describe here the use of surface UV illumination to record ethidium bromide-stained DNA gels with a video camera. This mode of illuminati on allows the use of a standard video camera equipped with a red filte r and results in a high signal strength. The assembly of a low-cost vi deo system on this basis is described. It uses tire public domain soft ware called Image on a Macintosh(R) computer and PostScript(TM) laser printer or a thermal printer to generate hard copies. The setup is sen sitive enough to detect 500 pg of DNA on an ethidium bromide-stained D NA gel. The UV illumination method described here can also greatly imp rove the sensitivity of existing video recording equipment.