Enterotoxin A was purified from 400 ml of Staphylococcus aureus strain
FRI-722 culture supernatant fluid. The sac culture method was used to
produce the enterotoxin. Ten milligrams (36%) of purified enterotoxin
A was obtained from the 28 mg present in the culture supernatant flui
d. The purified enterotoxin was homogeneous when analyzed by sodium do
decyl sulfate-polyacrylamide gel electrophoresis. The procedures used
are adequate for purifying a satisfactory amount of enterotoxin and ca
n be used in laboratories where the necessary equipment is not availab
le for handling large volumes of culture supernatant fluid.