PURIFICATION OF STAPHYLOCOCCAL-ENTEROTOXIN A BY DYE-LIGAND CHROMATOGRAPHY

Enterotoxin A was purified from 400 ml of Staphylococcus aureus strain FRI-722 culture supernatant fluid. The sac culture method was used to produce the enterotoxin. Ten milligrams (36%) of purified enterotoxin A was obtained from the 28 mg present in the culture supernatant flui d. The purified enterotoxin was homogeneous when analyzed by sodium do decyl sulfate-polyacrylamide gel electrophoresis. The procedures used are adequate for purifying a satisfactory amount of enterotoxin and ca n be used in laboratories where the necessary equipment is not availab le for handling large volumes of culture supernatant fluid.