IN-VITRO FERTILIZATION OF FOLLICULAR OOCYTES BY FROZEN-THAWED SPERMATOZOA IN JAPANESE MONKEYS (MACACA-FUSCATA)

We report the cryopreservation of epididymal spermatozoa and in vitro fertilization for Japanese monkeys (Macaca fuscata). Ovarian stimulati on of seven monkeys was studied by the administration of equine chorio nic gonadotrophin (eCG) and human chorionic gonadotrophin (hCG) during the nonbreeding season, after which oocytes were collected from ovari an follicles. The oocytes were subjected to in vitro fertilization by the frozen-thawed epididymal spermatozoa collected from a male monkey during the nonbreeding season. Before the experiment the epididymides and oocytes were transported about 500 km, during which the epididymid es were kept in mineral oil to prevent drying and the oocytes were kep t in a maturation medium. This technique of cryopreservation and in vi tro fertilization has a wide applicability in nonhuman primate reprodu ction, including the treatment of infertility and the preservation of wild, nonhuman primates.